# Mutaion Analysis

The Mutation Analysis tool of our web server is designed to predict the likelihood of break occurrences near a specified point mutation. This feature is crafted to provide insights in a user-friendly interface.

[Click here](https://proj.cse.cuhk.edu.hk/aihlab/ssblazer/#/pred_snp/) to use this function.

## Specify a Mutant Position

<figure><img src="https://291993907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fue7hNT9bnXKZlKTD9W0c%2Fuploads%2FR1isrABSrcOgbYeduFJm%2Fimage.png?alt=media&#x26;token=0388653e-d016-4af9-bc86-8392f424f86e" alt="" width="563"><figcaption></figcaption></figure>

You will need to provide the following details for the mutation analysis:

* **Assembly Version:** Select the reference genome assembly version that corresponds to your data.
* **Mutation Position:** Enter the specific genomic position (chromosome and position) of the mutation you are analyzing.
* **Alleles:** Indicate the alleles involved in the mutation, detailing both the wild-type and the mutated nucleotides.

## Get the Result

{% hint style="info" %}
The prediction process typically takes approximately 60 seconds.
{% endhint %}

<figure><img src="https://291993907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fue7hNT9bnXKZlKTD9W0c%2Fuploads%2FEYIIeQ6YIWurVu6tP84e%2Fimage.png?alt=media&#x26;token=448adfae-74d6-42a9-8316-6505c8a38875" alt="" width="563"><figcaption></figcaption></figure>

Once the process is completed, the results will be available on the results page.

SSBlazer predicts the probability of single-strand breaks within a range of -125 to +125 base pairs from the specified mutation position. Our genome browser interface will display these probabilities, contrasting the wild-type genome against the mutant genome for easy comparison and analysis.